KMID : 0923620200200050042
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Immune Network 2020 Volume.20 No. 5 p.42 ~ p.42
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CCAAT/enhancer binding protein ¥â Induces Post-Switched B Cells to Produce Blimp1 and Differentiate into Plasma Cells
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Lee Geon-Hee
Jang Eun-Kyeong Youn Jee-Hee
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Abstract
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Long-lasting post-switched plasma cells (PCs) arise mainly from germinal center (GC) reactions, but little is known about the mechanism by which GC B cells differentiate into PCs. Based on our observation that the expression of the transcription factor CCAAT/enhancer binding protein ¥â (C/EPB¥â) is associated with the emergence of post-switched PCs, we enquired whether a cell-autonomous function of C/EPB¥â is involved in the program for PC development. To address this, we generated C/EPB¥â-deficient mice in which the Cebpb locus was specifically deleted in B cells after transcription of the Ig ¥ã1 constant gene segment (C¥ã1). In response to in vitro stimulation, B cells from these Cebpbfl/flC¥ã1Cre/+ mice had defects in the induction of B lymphocyte-induced maturation protein 1 (Blimp1) and the formation of IgG1+ PCs, but not in proliferation and survival. At steady state, the Cebpbfl/flC¥ã1Cre/+ mice had reduced serum IgG1 titers but normal IgG2c and IgM titers. Moreover, upon immunization with T-dependent Ag, the mice produced reduced levels of Ag-specific IgG1 Ab, and were defective in the production of Ag-specific IgG1 Ab-secreting cells. These results suggest that a cell-autonomous function of C/EPB¥â is crucial for differentiation of post-switched GC B cells into PCs through a Blimp1-dependent pathway.
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KEYWORD
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Plasma cells, Immunoglobulin class switching, C/EBP¥â
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